Difference between revisions of "Part:BBa K3893030"
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===Usage and Biology===
 
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== Part engineering and DBTL cycle ==
  
 
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Revision as of 19:22, 19 October 2021


Population control device (QS-based lysis protein oscilator)

domestication.
Figure 1. SBOL Visual representation of the device

This device is an AHL induced transcriptional unit to express phiX174 lysis protein together with a TU for the constitutive expression of LuxI. It was assembled with a one-pot Level 2 Golden Gate reaction using BsmBI type IIS endonuclease.

This device is composed of the following standardized composite parts:

  • BBa_K3893028: a Level 2 transcriptional unit constitutively expressing luxI gene (Golden Braid compatible)
  • BBa_K3893029: a Level 2 transcriptional unit expresing phiX174 lysis protein under the control of the pux promoter (Golden Braid compatible)


Usage and Biology

Part engineering and DBTL cycle

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 956
    Illegal NheI site found at 979
    Illegal NheI site found at 1914
    Illegal NheI site found at 1937
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]