Difference between revisions of "Part:BBa K3805209"

Revision as of 13:01, 18 October 2021

Bi-stable Switch

The bistable switch is composed of trc promoter(BBa_K3805208), P2 promoter(BBa_I746104), lacI- LVA (BBa_C0012)and mcherry-ompt(BBa_K3805886).When AIP is released, it will act on P2 promoter and start the expression of lacI-LVA and downstream mcherry-ompt. LacI-LVA inhibits trc promoter.When the concentration of AIP decreases, the P2 promoter will be closed, and the inhibition of lacI-LVA on the trc promoter will be relieved.The trc promoter starts the expression of the target protein.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1287
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3805209 short</partinfo>
-The bistable switch is composed of trc promoter(BBa_K3805208), P2 promoter(BBa_I746104), lacI- LVA (BBa_C0012)and mcherry-ompt.When AIP is released, it will act on P2 promoter and start the expression of lacI-LVA and downstream mcherry-ompt. LacI-LVA inhibits trc promoter.When the concentration of AIP decreases, the P2 promoter will be closed, and the inhibition of lacI-LVA on the trc promoter will be relieved.The trc promoter starts the expression of the target protein.
+The bistable switch is composed of trc promoter(BBa_K3805208), P2 promoter(BBa_I746104), lacI- LVA (BBa_C0012)and mcherry-ompt(BBa_K3805886).When AIP is released, it will act on P2 promoter and start the expression of lacI-LVA and downstream mcherry-ompt. LacI-LVA inhibits trc promoter.When the concentration of AIP decreases, the P2 promoter will be closed, and the inhibition of lacI-LVA on the trc promoter will be relieved.The trc promoter starts the expression of the target protein.
 <!-- Add more about the biology of this part here