Difference between revisions of "Part:BBa K4088888"

Line 5: Line 5:
 
С-terminal fragment of β-lactamase fused with dCas13.<br/>
 
С-terminal fragment of β-lactamase fused with dCas13.<br/>
 
β-Lactamase (AmpR) - <br/>
 
β-Lactamase (AmpR) - <br/>
C-terminal fragment of β-lactamase - <br/>
+
C-terminal fragment of β-lactamase - <br/><br/><br/><br/>
dCAS13 - <br/><br/>
+
dCAS13 is a catalytically dead Cas13 enzyme. <br/><br/>
  
  

Revision as of 12:30, 18 October 2021


dCas13a-Clact

С-terminal fragment of β-lactamase fused with dCas13.
β-Lactamase (AmpR) -
C-terminal fragment of β-lactamase -



dCAS13 is a catalytically dead Cas13 enzyme.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1580
    Illegal BglII site found at 1790
    Illegal BglII site found at 2057
    Illegal BglII site found at 2822
    Illegal XhoI site found at 3751
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]