Difference between revisions of "Part:BBa K4083004"
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nadE from Pseudomonas aeruginosa is coding for NAD synthetase which synthesizes NAD+ from deamido-NAD+ by ATP-dependent amidation [1].
 
nadE from Pseudomonas aeruginosa is coding for NAD synthetase which synthesizes NAD+ from deamido-NAD+ by ATP-dependent amidation [1].
 
ATP + deamido-NAD+ + NH4+ -> AMP + diphosphate + H+ + NAD+
 
ATP + deamido-NAD+ + NH4+ -> AMP + diphosphate + H+ + NAD+
 +
 +
https://static.igem.org/mediawiki/parts/thumb/4/45/BBa_K4083004-NADS_reaction.png/799px-BBa_K4083004-NADS_reaction.png
  
 
Reference:
 
Reference:

Revision as of 12:26, 19 October 2021


nadE gene of Pseudomonas aeruginosa

nadE from Pseudomonas aeruginosa is coding for NAD synthetase which synthesizes NAD+ from deamido-NAD+ by ATP-dependent amidation [1]. ATP + deamido-NAD+ + NH4+ -> AMP + diphosphate + H+ + NAD+

799px-BBa_K4083004-NADS_reaction.png

Reference:

[1] UniProt. (n.d.). nadE - NH(3)-dependent NAD(+) synthetase - Pseudomonas aeruginosa (strain PA7) - nadE gene & protein. https://www.uniprot.org/uniprot/A6VD32

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 208
    Illegal XhoI site found at 475
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 400
    Illegal NgoMIV site found at 470
    Illegal NgoMIV site found at 691
  • 1000
    COMPATIBLE WITH RFC[1000]