Difference between revisions of "Part:BBa K3875006"

Latest revision as of 09:14, 18 October 2021

Usage and Biology

Design: Contained gene: trpE ( BBa_K3875009), trpG ( BBa_K3875003), trpC ( BBa_K3875002).
This part could express two enzymes, indole-3-glycerol-phosphate synthase (IGPS, encoded by trpC) and anthranilate synthase (AS, encoded by trpE).
Erythritos-4-phosphate (E4P) from the pentose phosphate pathway (PPP) is condensed with phosphoenolpyruvate (PEP) from the glycolysis pathway (EMP) to form 3-deoxy-D-arabinheptanose-7-phosphate (DAHP).
After 7 steps, DAHP is converted to chorismate. Our trpE and trpC genes in E. coli promote the chorismate into the latter half of the 5-HTP synthesis pathway (Figure 1).






Below is plasmid profile of pSA74-lac-trpE-trpG-AntrpC-T1




Method: The pcd and p4h genes were amplified by PCR, then the two genes and pSA74 were digested by restriction endonuclease, and finally pSA74-lac-trpE-trpG-AntrpC-T1 was obtained by ligase T4. Then HindIII and BamHI were used for digestion verification.
Result: The result of agarose gel electrophoresis proves that our plasmid has been successfully constructed .

Sequence and Features