Difference between revisions of "Part:BBa K3927020"
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===Design=== | ===Design=== | ||
[[File:T--NUS Singapore--pGH3CF.png|700px|thumb|center|pGH3CF construct schematic]] | [[File:T--NUS Singapore--pGH3CF.png|700px|thumb|center|pGH3CF construct schematic]] | ||
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+ | To test out the functionality of the driver circuit, a construct with 3C120-CYC-LacO controlling the FLO1 gene was designed (figure 1). Anticipating the need to test integration, this plasmid was also designed to include the HBD2 gene under the control of the GAL1 promoter. | ||
===Usage=== | ===Usage=== |
Revision as of 10:27, 18 October 2021
3C120-CYC-LacO-Flo1
3C120-CYC-LacO controlling of expression such that in the presence of blue light(dependent on EL222 expression) AND NOT LacI, yeast express the flocculation phenotype.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 203
Illegal SpeI site found at 535 - 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 185
Illegal SpeI site found at 535 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 203
Illegal SpeI site found at 535 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 203
Illegal SpeI site found at 535
Illegal AgeI site found at 439
Illegal AgeI site found at 481
Illegal AgeI site found at 616
Illegal AgeI site found at 886 - 1000COMPATIBLE WITH RFC[1000]
Description
Design
To test out the functionality of the driver circuit, a construct with 3C120-CYC-LacO controlling the FLO1 gene was designed (figure 1). Anticipating the need to test integration, this plasmid was also designed to include the HBD2 gene under the control of the GAL1 promoter.
Usage
Characterization