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σs have been divided into four groups based on their phylogenetic relationships and modular structure.σ28, a Group 3 σ, is the most widely distributed alternative σ factor, making it an attractive candidate for study of its promoter recognition. σ28 controls expression of flflagella-related genes in all motile Gram-negative and Gram-positive bacteria, and plays a role in development in some non-motile bacteria.<br> | σs have been divided into four groups based on their phylogenetic relationships and modular structure.σ28, a Group 3 σ, is the most widely distributed alternative σ factor, making it an attractive candidate for study of its promoter recognition. σ28 controls expression of flflagella-related genes in all motile Gram-negative and Gram-positive bacteria, and plays a role in development in some non-motile bacteria.<br> | ||
Some researches found very similar results for σ28 RNA polymerase from C. trachomatis and E. coli, suggesting that promoter recognition by this alternative RNA polymerase is well conserved among bacteria. Bioinformatic analysis of E. coli s28 promoters suggests that their consensus sequence is TAAAgttt-N11-GCCGATAA. | Some researches found very similar results for σ28 RNA polymerase from C. trachomatis and E. coli, suggesting that promoter recognition by this alternative RNA polymerase is well conserved among bacteria. Bioinformatic analysis of E. coli s28 promoters suggests that their consensus sequence is TAAAgttt-N11-GCCGATAA. |
Revision as of 06:19, 18 October 2021
σ28 (fliA)
σ28 is a special transcriptional factor concerning the fliA, which is an alternate sigma factor for the class 3 flagella operons.
Usage and Biology
![](https://static.igem.org/mediawiki/parts/1/1f/124_tu1.png)
Figure 1. Sequence logos for the -35 and -10 elements of the σ28-dependent promoter. (A) Sequence recognized by C. trachomatis σ28 RNA polymerase in the context of the C. trachomatis hctB promoter. (B) Sequence recognized by E. coli σ28 RNA polymerase in the same promoter context. (C) The sequence logo based on the nucleotide frequencies of known bacterial σ28 promoters.
Some researches found very similar results for σ28 RNA polymerase from C. trachomatis and E. coli, suggesting that promoter recognition by this alternative RNA polymerase is well conserved among bacteria. Bioinformatic analysis of E. coli s28 promoters suggests that their consensus sequence is TAAAgttt-N11-GCCGATAA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 426
Illegal SapI.rc site found at 451
Illegal SapI.rc site found at 607