Difference between revisions of "Part:BBa K3759017"
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<partinfo>BBa_K3759017 short</partinfo> | <partinfo>BBa_K3759017 short</partinfo> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3759017 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3759017 SequenceAndFeatures</partinfo> | ||
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| + | <!-- Uncomment this to enable Functional Parameter display | ||
| + | ===Functional Parameters=== | ||
| + | <partinfo>BBa_K3759017 parameters</partinfo> | ||
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LCC is a leaf-branch compost cutinase[1] and a kinetically robust protein[2]. A research published on Nature came up with a mutant enzyme, mLCC[1] that hydrolyzes 90% of PET in plastic bottles in just 10 hours. This is more efficient than any previous PET hydrolase, and more importantly, the resulting monomers- ethylene glycol and terephthalic acid have the same properties as the monomers found in petrochemical materials. | LCC is a leaf-branch compost cutinase[1] and a kinetically robust protein[2]. A research published on Nature came up with a mutant enzyme, mLCC[1] that hydrolyzes 90% of PET in plastic bottles in just 10 hours. This is more efficient than any previous PET hydrolase, and more importantly, the resulting monomers- ethylene glycol and terephthalic acid have the same properties as the monomers found in petrochemical materials. | ||
| − | The linker is | + | The linker is GGGGSGGGGS. |
Hydrophobin could be produced by filamentous fungi, such as Ascomycetes and Basidiomycetes. Many different aspects of fungal development have been attributed to hydrophobin. For example, they are thought to play a role in the formation of aerial hyphae and fruiting bodies. One of the most important features of hydrophobin is that they are able to assemble spontaneously into amphipathic monolayers at hydrophobic–hydrophilic interfaces. | Hydrophobin could be produced by filamentous fungi, such as Ascomycetes and Basidiomycetes. Many different aspects of fungal development have been attributed to hydrophobin. For example, they are thought to play a role in the formation of aerial hyphae and fruiting bodies. One of the most important features of hydrophobin is that they are able to assemble spontaneously into amphipathic monolayers at hydrophobic–hydrophilic interfaces. | ||
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The construction includes: | The construction includes: | ||
| − | 1. a 6× His tag | + | 1. a 6× His tag is added to enable us carrying out Ni-NTA protein purification. |
===Protein Expression=== | ===Protein Expression=== | ||
Revision as of 03:14, 20 October 2021
mLCC-linker-mHFBI
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 187
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 196