Difference between revisions of "Part:BBa P0140"
Line 14: | Line 14: | ||
<partinfo>TetR BBa_P0140</partinfo> | <partinfo>TetR BBa_P0140</partinfo> | ||
− | + | Tianjin 2021's characterization | |
− | <b>< | + | <b><h2>New information of TetR operon leakage</h2></b> |
<p >Group: Tianjin 2021<br> | <p >Group: Tianjin 2021<br> | ||
Author: Ruiqi Liu<br> | Author: Ruiqi Liu<br> |
Revision as of 01:39, 18 October 2021
PoPS -> TetR [S0163]
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
No part name specified with partinfo tag.
Tianjin 2021's characterization
New information of TetR operon leakage
Group: Tianjin 2021
Author: Ruiqi Liu
Summary: we added information about TetR operon leakage.
Background
In order to better apply the tetR operon in the project, we characterized the leakage amount of the tetR operon. We constructed a carotene plasmid with tetR operon, and transformed it into yeasts, then cultured the yeasts without Atc inducer. By observing the color of the colony, the leakage of tetR operon can be roughly determined.
Result
Our experimental results show that the tetR operon is very easy to leak, and the expression amount of the circuit is different and difficult to test. Therefore, the results clearly indicated that in strict experiments, using the tetR operon alone will affect the experimental results.
Design