Difference between revisions of "Part:BBa K3738008:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal histidine tag and has had the polyarginine tag removed. | ||
| + | |||
This part has not yet been codon-optimized. | This part has not yet been codon-optimized. | ||
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| − | |||
===Source=== | ===Source=== | ||
Revision as of 03:23, 22 October 2021
MS2 Bacteriophage Capsid Protein Dimer
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 138
Illegal PstI site found at 546 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 138
Illegal PstI site found at 546 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 138
Illegal PstI site found at 546 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 138
Illegal PstI site found at 546 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is an improved version of the part BBa_K2683012, and has been modified to include an N-terminal histidine tag and has had the polyarginine tag removed.
This part has not yet been codon-optimized.
Source
https://doi.org/10.1111/mmi.14406
References
de Martín Garrido, N., Crone, M.A., Ramlaul, K., Simpson, P.A., Freemont, P.S. and Aylett, C.H.S. (2020), Bacteriophage MS2 displays unreported capsid variability assembling T = 4 and mixed capsids. Mol Microbiol, 113: 143-152. https://doi.org/10.1111/mmi.14406