Difference between revisions of "Part:BBa K3738002:Design"
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===Design Notes=== | ===Design Notes=== | ||
| + | This part is a modified version of BBa_K3738000, to which an C-terminal histidine tag has been added. As maturation protein A has not been well studied for use in phage-like particles, we have designed various versions of the part for testing purposes. | ||
| + | |||
This part has been codon optimized for use in E.coli. | This part has been codon optimized for use in E.coli. | ||
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===Source=== | ===Source=== | ||
Revision as of 01:41, 18 October 2021
MS2 Bacteriophage Maturation Protein A with C-Terminal Histidine Tag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 836
Illegal XhoI site found at 919 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 925
Design Notes
This part is a modified version of BBa_K3738000, to which an C-terminal histidine tag has been added. As maturation protein A has not been well studied for use in phage-like particles, we have designed various versions of the part for testing purposes.
This part has been codon optimized for use in E.coli.
Source
Source: https://www.uniprot.org/uniprot/P03610
References
Meng, R., Jiang, M., Cui, Z. et al. Structural basis for the adsorption of a single-stranded RNA bacteriophage. Nat Commun 10, 3130 (2019). https://doi.org/10.1038/s41467-019-11126-8