Difference between revisions of "Part:BBa K3941005"
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This composite part originated from a codon-optimized (for E. coli DH5⍺) version of the endoglucanase (EG) gene that cleaves the internal beta-1,4-glycosidic bonds in cellulose. We optimized the sequence for expression and added a 6XHis at the end. | This composite part originated from a codon-optimized (for E. coli DH5⍺) version of the endoglucanase (EG) gene that cleaves the internal beta-1,4-glycosidic bonds in cellulose. We optimized the sequence for expression and added a 6XHis at the end. | ||
Revision as of 21:04, 17 October 2021
pET29b+EGII
BBa_K3941005 is a composite part that we used in our expression plasmids (pET29b). We obtain EG2 wild type with this sequence.
This composite part contains 4 parts:
- T7 Promoter (BBa_K3941000)
- LacO (BBa_K1624002)
- EGII (BBa_K3941001)
- T7 Terminator
EGII
This composite part originated from a codon-optimized (for E. coli DH5⍺) version of the endoglucanase (EG) gene that cleaves the internal beta-1,4-glycosidic bonds in cellulose. We optimized the sequence for expression and added a 6XHis at the end. This part contains a mutation to form a different amino acid than wild type. We changed the Cystine from the 99th amino acid to Valine.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 22
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 93
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 22
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 22
- 1000COMPATIBLE WITH RFC[1000]