Difference between revisions of "Part:BBa K3771075"
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<img src="https://2021.igem.org/wiki/images/4/4c/T--NCKU_Tainan--taurine_pathway.png" style="width:35%;"> | <img src="https://2021.igem.org/wiki/images/4/4c/T--NCKU_Tainan--taurine_pathway.png" style="width:35%;"> | ||
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<br><b style="font-size:1.3rem">Usage</b> | <br><b style="font-size:1.3rem">Usage</b> | ||
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+ | <br>CDO1 was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC). <br> | ||
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+ | <div style="width=100%; display:flex; align-items: center; justify-content: center;"> | ||
+ | <img src="https://2021.igem.org/wiki/images/a/aa/T--NCKU_Tainan--3.png" style="width:35%;"> | ||
+ | </div> | ||
+ | <p>Fig. 5 Taurine production of Ptrc-cdo1 +PlacI-csad +Ptrc-cs in different growth mediums.</p> | ||
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+ | <div style="width=100%; display:flex; align-items: center; justify-content: center;"> | ||
+ | <img src="https://2021.igem.org/wiki/images/6/6b/T--NCKU_Tainan--invivo1.png" style="width:35%;"> | ||
+ | </div> | ||
+ | <p>Fig. 6 Taurine production of Ptrc-cdo1+Ptrc-csad in different growth mediums.</p> | ||
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+ | <div style="width=100%; display:flex; align-items: center; justify-content: center;"> | ||
+ | <img src="https://2021.igem.org/wiki/images/6/6b/T--NCKU_Tainan--invivo1.png" style="width:35%;"> | ||
+ | </div> | ||
+ | <p>Fig. 7 Taurine production of Ptrc-cdo1+Ptrc-csad in different growth mediums.</p> | ||
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− | <br><b style="font-size:1.3rem">Characterization</b> | + | |
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+ | <br><b style="font-size:1.3rem">Characterization</b> | ||
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Revision as of 11:20, 18 October 2021
Ptrc-CDO1
Description
Ptrc-cdo1 is a composite part consisting of the trc promoter and the cdo1 sequences. This part was used in in vivo testing of taurine production. The sequence for cdo1 and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).
Biology
Trc promoter constitutively facilitates the expression of CDO1 enzyme.
Fig. 1 Confirmation of cdo1 fragment by PCR. M: Marker; Lane 1: cdo1 (603 bp)
Fig. 2 Confirmation of pSB4KI-Ptrc-CDO1 by digestion. M: Marker; Lane 1~3: Different colonies of pSB4KI-Ptrc-CDO1 (5470 bp)
Fig. 3 Transformation / CDO1 in DH5α
Fig. 4 Confirmation of protein expression of CDO1. M: Marker; Lane1: CDO1 in DH5α without induction; Lane2: CDO1 in DH5α with induction (~22 kDa)
Usage
CDO1 was used in in vivo testing of taurine production. The sequence for CDO1 enzyme and trc promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).
Fig. 5 Taurine production of Ptrc-cdo1 +PlacI-csad +Ptrc-cs in different growth mediums.
Fig. 6 Taurine production of Ptrc-cdo1+Ptrc-csad in different growth mediums.
Fig. 7 Taurine production of Ptrc-cdo1+Ptrc-csad in different growth mediums.
Characterization
References
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 534
- 1000COMPATIBLE WITH RFC[1000]