Difference between revisions of "Part:BBa K3771028"

Revision as of 18:52, 17 October 2021 (view source) Marvinang02 (Talk | contribs) ← Older edit		Revision as of 20:55, 19 October 2021 (view source) Evelynnn (Talk | contribs) Newer edit →
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−	<br>We ligased the pLacI-OmpA* fragment and pspA-CoaBC on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.	+	<br>We ligased the <i>P<sub>lacI</sub>-ompA/oprF</i> fragment and <i>P<sub>pspA</sub>-coaBC</i> on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.
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Revision as of 20:55, 19 October 2021

PpspA-CoaBC-PlacI-OmpA/OprF

Description

This composite part is a component of the IFN-γ sensing system and was used to express the taurine production enzyme, CoaBC.

Biology

The LacI promoter facilitates the constitutive expression of OmpA/OprF. Binding of IFN-γ to the OmpA/OprF chimeric protein induces the response of the phage shock protein (Psp) system, a highly conserved stress response system in enterobacteria[1]. Signal transduction from the outer membrane to the inner membrane activates the pspA promoter, initiating expression of CoaBC. CoaBC converts L-cystate into taurine in the taurine synthesis L-cystate pathway.

Usage

We ligased the P_lacI-ompA/oprF fragment and P_pspA-coaBC on the pSU expression vector and transformed it into DH5α to complete construction of the plasmid.

Characterization

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References

1. Darwin AJ. The phage-shock-protein response. Molecular Microbiology. 2005;57(3):621-628. doi:10.1111/j.1365-2958.2005.04694.x https://pubmed.ncbi.nlm.nih.gov/16045608/
Sequence and Features