Difference between revisions of "Part:BBa K3733032:Experience"
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HanZhenhao (Talk | contribs) (→Applications of BBa_K3733032) |
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===Applications of BBa_K3733032=== | ===Applications of BBa_K3733032=== | ||
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− | We transform <i>E.coli</i> DH5α strain with BBa_K3733032 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the | + | We transform <i>E.coli</i> DH5α strain with BBa_K3733032 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD<sub>600</sub> in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (<b>Figure 1</b>). |
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Latest revision as of 17:44, 17 October 2021
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Applications of BBa_K3733032
We transform E.coli DH5α strain with BBa_K3733032 and chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the report. The strain was expanded in LB medium to OD=0.4, then 198μL of bacterial solution was injected into a 96-well plate, and a series of concentration gradients (0mM, 0.001mM, 0.01mM, 0.1mM, 1mM) of sodium thiosulfate were added into each well to 200μL. Measure the fluorescence with gain of 75 and OD600 in the Synergy H1 microplate reader overnight. The results of our experiment are shown in the figure below (Figure 1).
![](https://static.igem.org/mediawiki/parts/6/6f/T--HZAU-China--103-1.png)
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