Difference between revisions of "Part:BBa K3776009"
Line 41: Line 41:
 
<p align="justify">
 
<p align="justify">
  
−
This shuttle vector was modified based on the shuttle vector <a href="https://parts.igem.org/Part:BBa_K3228089"> BBa_K3228089</a> designed by iGEM Team Marburg 2019.
+
This shuttle vector was modified based on the shuttle vector <a href="https://parts.igem.org/Part:BBa_K3228089"> BBa_K3228089</a> designed by iGEM Team Marburg 2019. It contains the ori ColE1 for E.coli and the minimal replication region of the pANS plasmid for S.elongatus. The minimal replication region of the pANS plasmid has been codon optimized for S.elongatus UTEX 2973 (see <a href="https://parts.igem.org/Part:BBa_K3776007"> BBa_K3776007</a>)
  
  

Revision as of 15:19, 17 October 2021


panS-loxP-MCS

This shuttle vector was modified based on the shuttle vector BBa_K3228089 designed by iGEM Team Marburg 2019. It contains the ori ColE1 for E.coli and the minimal replication region of the pANS plasmid for S.elongatus. The minimal replication region of the pANS plasmid has been codon optimized for S.elongatus UTEX 2973 (see BBa_K3776007)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 33
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 206
    Illegal BamHI site found at 6
    Illegal XhoI site found at 5663
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2378
    Illegal AgeI site found at 4103
  • 1000
    COMPATIBLE WITH RFC[1000]