Difference between revisions of "Part:BBa K3861021"
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This is the native promoter of the <i>Salmonella</i> typhimurium araBAD inducer system. P<sub>BAD</sub> initiates transcription during high L-arabinose and low glucose levels, as P<sub>BAD</sub> forms a complex with L-arabinose (Guzman et al., 1995). | This is the native promoter of the <i>Salmonella</i> typhimurium araBAD inducer system. P<sub>BAD</sub> initiates transcription during high L-arabinose and low glucose levels, as P<sub>BAD</sub> forms a complex with L-arabinose (Guzman et al., 1995). | ||
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| + | As a part of our project "TargetTaxi", we aim to use this promoter to regulate the expression of the SPI-1 master regulator, hilA. Thereby, allowing the overexpression of SPI-1 type 3 secretion machinery and its assembly at the cell poles. The presence of the machinery at the cell poles will result in its presence in the membrane of minicells. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 18:18, 20 October 2021
araBAD promoter from Salmonella Typhimurium
This is the native promoter of the Salmonella typhimurium araBAD inducer system. PBAD initiates transcription during high L-arabinose and low glucose levels, as PBAD forms a complex with L-arabinose (Guzman et al., 1995).
As a part of our project "TargetTaxi", we aim to use this promoter to regulate the expression of the SPI-1 master regulator, hilA. Thereby, allowing the overexpression of SPI-1 type 3 secretion machinery and its assembly at the cell poles. The presence of the machinery at the cell poles will result in its presence in the membrane of minicells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]