Difference between revisions of "Part:BBa K3750000"

 
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In C. reinhardtii, GSR is present in all cellular compartments. This part documents the cytosolic isoform of GSR in C. reinhardtii which is encoded by the gene GSHR1 (Merchant et al., 2007).
 
In C. reinhardtii, GSR is present in all cellular compartments. This part documents the cytosolic isoform of GSR in C. reinhardtii which is encoded by the gene GSHR1 (Merchant et al., 2007).
  
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<img src="https://parts.igem.org/File:T--UNSW_Australia--GSHR1.png">
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 05:49, 21 October 2021


GSHR1 glutathione reductase (from Chlamydomonas reinhardtii)

Glutathione reductase (GSR) is a homodimeric flavoprotein (50-kDa subunits). When Glutathione (GSH) neutralises ROS, GSH becomes a radical in the form of oxidised glutathione disulfide (GSSG). GSR regulates cellular glutathione homeostasis by using NADPH and FAD to catalyze the reduction of oxidised glutathione disulfide (GSSG) into glutathione. GSR is highly conserved in aerobic organisms as it is important for cell survival when the organism is subjected to oxidative stress (Carlberg, 1985). In C. reinhardtii, GSR is present in all cellular compartments. This part documents the cytosolic isoform of GSR in C. reinhardtii which is encoded by the gene GSHR1 (Merchant et al., 2007).

<img src="https://parts.igem.org/File:T--UNSW_Australia--GSHR1.png">

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 556
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 556
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 556
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 556
    Illegal NgoMIV site found at 145
    Illegal NgoMIV site found at 252
  • 1000
    COMPATIBLE WITH RFC[1000]