Difference between revisions of "Part:BBa K3815004"
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<partinfo>BBa_K3815004 short</partinfo>
 
<partinfo>BBa_K3815004 short</partinfo>
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<h3><font size="4.5">Descriotion of this part</font> </h3>
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<h3><font size="3">Targeted protein</font> </h3>
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This part is for the purfication of the small peptide, NOP1. It can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene that promotes the wilting of a plant and aging of cut flowers.<br><br>
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<h3><font size="3">Purification system</font> </h3>
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In order to purify the peptide, we adopted ELK16 system that the past iGEM teams had not used. This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein.<br>
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This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein. In this part, the targeted protein is NOP1.This is a small peptide that can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene and aging of cut flowers.
 
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  

Revision as of 10:03, 20 October 2021


NOP1-Mxe GryA intein-PT-linker-ELK16

Descriotion of this part

Targeted protein

This part is for the purfication of the small peptide, NOP1. It can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene that promotes the wilting of a plant and aging of cut flowers.

Purification system

In order to purify the peptide, we adopted ELK16 system that the past iGEM teams had not used. This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein.


Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 117
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 117
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 117
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 117
    Illegal NgoMIV site found at 550
  • 1000
    COMPATIBLE WITH RFC[1000]