Difference between revisions of "Part:BBa K3815007"
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<partinfo>BBa_K3815036 short</partinfo>
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<partinfo>BBa_K3815007 short</partinfo>
  
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This part is used His tag to purify the peptides. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT, we cut the N terminal of Mxe GyrA intein. Then, we can get the purpose protein.In this part, the purpose protein is Defencine1 derived from a honey bee. This is antimicrobial peptide that can inhibit the growth of gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.
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This part is made of Hist tag for the peptide production. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the targeted protein is Defencine1 derived from a honey bee. This is antimicrobial peptide that can inhibit the growth of gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.
  
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3815036 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3815007 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3815036 parameters</partinfo>
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<partinfo>BBa_K3815007 parameters</partinfo>
 
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Revision as of 09:19, 17 October 2021


Defensin1-Mxe GryA intein-PT-linker-His tag

This part is made of Hist tag for the peptide production. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the targeted protein is Defencine1 derived from a honey bee. This is antimicrobial peptide that can inhibit the growth of gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 249
    Illegal SpeI site found at 573
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 249
    Illegal SpeI site found at 573
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 249
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 249
    Illegal SpeI site found at 573
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 249
    Illegal SpeI site found at 573
    Illegal NgoMIV site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]