Difference between revisions of "Part:BBa K3815006"
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<partinfo>BBa_K3815035 short</partinfo>
 
<partinfo>BBa_K3815035 short</partinfo>
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This part is used His tag to purify the peptides. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT, we cut the N terminal of Mxe GyrA intein. Then, we can get the purpose protein.In this part, the purpose protein is CecropinA derived from a moth. This is the antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. This sequence got from the moth. In our experiment, we used it to kill the bacteria in the vase water.
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This part is made of His tag for peptide production. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the targeted protein is CecropinA derived from a moth. This is the antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. This sequence got from the moth. In our experiment, we used it to kill the bacteria in the vase water.
  
 
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Revision as of 09:29, 17 October 2021


No part name specified with partinfo tag. This part is made of His tag for peptide production. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the targeted protein is CecropinA derived from a moth. This is the antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. This sequence got from the moth. In our experiment, we used it to kill the bacteria in the vase water.

Sequence and Features No part name specified with partinfo tag.