Difference between revisions of "Part:BBa K3930011"
 
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<h2>Introduction</h2>
 
<h2>Introduction</h2>
 
<p>The HygR cassette is composed by the promoter ADH1, the resistance gene <i>hygR</i> and the transcription terminator of VPS13. The resistance gene is based on the part <a href="https://parts.igem.org/Part:BBa_K2386004" class="pr-0" target="_blank">(BBa_K2386004)</a>, but it was codon optimized for expression into <i>S. cerevisiae</i>. </p>
 
<p>The HygR cassette is composed by the promoter ADH1, the resistance gene <i>hygR</i> and the transcription terminator of VPS13. The resistance gene is based on the part <a href="https://parts.igem.org/Part:BBa_K2386004" class="pr-0" target="_blank">(BBa_K2386004)</a>, but it was codon optimized for expression into <i>S. cerevisiae</i>. </p>
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<p>This gene codes for a hygromycin phosphotransferase, which inactivates the hygromycin by adding a phosphate on it. Therefore, this cassette allows for the selection of yeast transformants upon addition of Hygromycin into the media of culture. </p>  
+
<p>This gene codes for a hygromycin phosphotransferase, which inactivates the hygromycin by adding a phosphate on it. Therefore, this cassette allows for the selection of yeast transformants upon addition of hygromycin into the media of culture. </p>  
  
 
<h3>Part characterization</h3>
 
<h3>Part characterization</h3>

Latest revision as of 09:01, 17 October 2021


Hygromycin selective cassette Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1042
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1042
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1042
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1042
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 90

Introduction

The HygR cassette is composed by the promoter ADH1, the resistance gene hygR and the transcription terminator of VPS13. The resistance gene is based on the part (BBa_K2386004), but it was codon optimized for expression into S. cerevisiae.

This gene codes for a hygromycin phosphotransferase, which inactivates the hygromycin by adding a phosphate on it. Therefore, this cassette allows for the selection of yeast transformants upon addition of hygromycin into the media of culture.

Part characterization

The part BBa_K3930011 was used to select the genomic integration of the part (BBa_K3930000). Transformants were plated onto YPD + 200 μg.ml-1 hygromycin Petri dish. The Toulouse INSA UPS team managed to select transformants with this selection marker (for more details, check the BBa_K3930000 part page).