Difference between revisions of "Part:BBa K3815005"
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− | This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the | + | This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of the ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein. In this part, the targeted protein is NOP1v.This is a small peptide that can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene and aging of cut flowers. We could not get NOP1 sufficiently when using BBa_K3815033, so we added a valine to the N-terminus of NOP1 according to N-end-rule. |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
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Revision as of 09:26, 17 October 2021
NOP1v-Mxe GryA intein-PT-linker-ELK16
This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of the ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein. In this part, the targeted protein is NOP1v.This is a small peptide that can prevent the plants from accepting ethylene. In our experiment, we used it to inhibit the acceptance of ethylene and aging of cut flowers. We could not get NOP1 sufficiently when using BBa_K3815033, so we added a valine to the N-terminus of NOP1 according to N-end-rule.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 120
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 120
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 120
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 120
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 120
Illegal NgoMIV site found at 553 - 1000COMPATIBLE WITH RFC[1000]