Difference between revisions of "Part:BBa K3771010"
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<br><br>In our project, the sequence of OmpA protein was used to construct the OmpA/OprF chimeric protein. | <br><br>In our project, the sequence of OmpA protein was used to construct the OmpA/OprF chimeric protein. | ||
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Revision as of 07:45, 17 October 2021
Outer Membrane Protein A (OmpA)
Description
Outer membrane protein A (OmpA) is a 35 kDa transmembrane protein found in many enterobacteria. It is responsible for maintaining the stability of the bacterial membrane and cell signaling. [1]
Biology
In E. coli, the β-barrel conformation of OmpA is composed of extracellular loops that help play a role in the detection of outer membrane stress and binding of extracellular molecules. OmpA also serves as a porin channel that regulates water transport within the bacterial cell. [2]
Usage
In our project, the sequence of OmpA protein was used to construct the OmpA/OprF chimeric protein.
<a href="https://2021.igem.org/File:T--NCKU_Tainan--ompA_oprF.gif" target="_blank" style="width:60%"><img src="https://2021.igem.org/File:T--NCKU_Tainan--ompA_oprF.gif" alt="" title="" style="width:100%"></a>
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 881
Illegal BamHI site found at 744 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]