Difference between revisions of "Part:BBa K3784000"
(→‎Characterization)
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===Characterization===
 
===Characterization===
 
We linked acdA fragment and pET-28a plasmid, then transferred the recombinant plasmid into E. coli and test it by colony PCR.
 
We linked acdA fragment and pET-28a plasmid, then transferred the recombinant plasmid into E. coli and test it by colony PCR.
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[[File:T--bnuz_china--dianyong.jpeg]]
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[[File:BNUZ-acda.png]]
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 07:24, 17 October 2021


acdA (encoding 3-(aryl) acrylate reductase)

acdA is from the FLD gene cluster of Clostridium sporogenes, involved in the conversion of tryptophan to 3-Indolepropionic acid. In the five-step metabolic pathway we reconstructed, acdA encodes 3-(aryl)acrylate reductase, which catalyze indole acrylic acid to produce indole propionic acid.

Characterization

We linked acdA fragment and pET-28a plasmid, then transferred the recombinant plasmid into E. coli and test it by colony PCR. BNUZ-acda.png

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 291
    Illegal SpeI site found at 619
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 291
    Illegal SpeI site found at 619
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1141
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 291
    Illegal SpeI site found at 619
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 291
    Illegal SpeI site found at 619
  • 1000
    COMPATIBLE WITH RFC[1000]