Difference between revisions of "Part:BBa K3729002"
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Beta-hydroxybutyryl-CoA dehydrogenase catalyzes an important step (acetyl-CoA to 3-hydroxybutyryl-CoA) in the acetyl-CoA butyrate synthesis pathway, further increasing the efficiency of bacteria’s butyrate production. We obtained the DNA sequence (HBD) encoded for butyryl- CoA dehydrogenase from the genome of E. coli Nissle 1917. | Beta-hydroxybutyryl-CoA dehydrogenase catalyzes an important step (acetyl-CoA to 3-hydroxybutyryl-CoA) in the acetyl-CoA butyrate synthesis pathway, further increasing the efficiency of bacteria’s butyrate production. We obtained the DNA sequence (HBD) encoded for butyryl- CoA dehydrogenase from the genome of E. coli Nissle 1917. | ||
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| + | <b>Construct design:</b>Construct design: We optimized the DNA sequence (HBD gene) for expression in E. coli, inserting it into pet-28a, one of the most common, popular expression plasmid for experiments. This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of beta hydroxybutyryl CoA dehydrogenase. Our composite part is synthesized by IDT. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Revision as of 17:44, 21 October 2021
HBD (Beta-hydroxybutyryl CoA dehydrogenase)
Beta-hydroxybutyryl-CoA dehydrogenase catalyzes an important step (acetyl-CoA to 3-hydroxybutyryl-CoA) in the acetyl-CoA butyrate synthesis pathway, further increasing the efficiency of bacteria’s butyrate production. We obtained the DNA sequence (HBD) encoded for butyryl- CoA dehydrogenase from the genome of E. coli Nissle 1917.
Construct design:Construct design: We optimized the DNA sequence (HBD gene) for expression in E. coli, inserting it into pet-28a, one of the most common, popular expression plasmid for experiments. This design of construct would lead to overexpression of the DNA sequence, further upregulating the production of beta hydroxybutyryl CoA dehydrogenase. Our composite part is synthesized by IDT.