Difference between revisions of "Part:BBa K3740004"
(→‎2021 SZPT-China)
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<h3>References</h3>
 
<h3>References</h3>
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<p>[1] Gupta S, Bram E E, Weiss R. Genetically programmable pathogen sense and destroy. [J]. Acs Synthetic Biology, 2013, 2(12):715-723.
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<p>[1] Gupta S, Bram E E, Weiss R. Genetically programmable pathogen sense and destroy. [J]. Acs Synthetic Biology, 2013, 2(12):715-723.</p>
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[2] Michel-Briand Y, Baysse C. The pyocins of Pseudomonas aeruginosa. [J]. Biochimie, 2002, 84(5-6):499-510</p>
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<p>[2] Michel-Briand Y, Baysse C. The pyocins of Pseudomonas aeruginosa. [J]. Biochimie, 2002, 84(5-6):499-510</p>

Revision as of 02:27, 17 October 2021


Translocationl domain of Pyocin S2

Description

Involves in the translocation across the outer membrane,we used it to construct chimeric bacteriocin SE.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 732
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 531
    Illegal AgeI site found at 621
  • 1000
    COMPATIBLE WITH RFC[1000]


2021 SZPT-China

Biology

Bacteriocin activity proteins have three distinct domains arranged from N to C terminus that include receptor,translocase,and nuclease (with immunity protein-binding)domains. The translocation domain are species specific.


References

[1] Gupta S, Bram E E, Weiss R. Genetically programmable pathogen sense and destroy. [J]. Acs Synthetic Biology, 2013, 2(12):715-723.

[2] Michel-Briand Y, Baysse C. The pyocins of Pseudomonas aeruginosa. [J]. Biochimie, 2002, 84(5-6):499-510