Difference between revisions of "Part:BBa K3791007"
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Here we post the full guide RNA that allows for targeting a specific erythromycin-resistance gene by using CRISPR-LbCas12a system. That means, a repeat sequence, needed by the gRNA to couple to the Cas12 protein, (<html><a href="https://parts.igem.org/Part:BBa_K2927006" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K2927006</a></html>) and a spacer sequence which is specific for the desired gene to target (<html><a href="https://parts.igem.org/Part:BBa_K3791002" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K3791002</a></html>).
 
Here we post the full guide RNA that allows for targeting a specific erythromycin-resistance gene by using CRISPR-LbCas12a system. That means, a repeat sequence, needed by the gRNA to couple to the Cas12 protein, (<html><a href="https://parts.igem.org/Part:BBa_K2927006" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K2927006</a></html>) and a spacer sequence which is specific for the desired gene to target (<html><a href="https://parts.igem.org/Part:BBa_K3791002" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K3791002</a></html>).
  
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===Usage and Biology===
 
  
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===Usage, Biology and Characterization===
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As this part is a simplification of our final construct, usage, biology and characterization is explained there: <html><a href="https://parts.igem.org/Part:BBa_K3791011" target="_blank" onmouseover="this.style.color='#ff724a';" onmouseout="this.style.color='#ff9515';" style="color: #ff9515; text-decoration: none;">BBa_K3791011</a></html>
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<span class='h3bb'><b>Sequence and Features</b></span>
 
<span class='h3bb'><b>Sequence and Features</b></span>
 
<partinfo>BBa_K3791007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3791007 SequenceAndFeatures</partinfo>

Revision as of 01:04, 20 October 2021


gRNA Erythromycin

Here we post the full guide RNA that allows for targeting a specific erythromycin-resistance gene by using CRISPR-LbCas12a system. That means, a repeat sequence, needed by the gRNA to couple to the Cas12 protein, (BBa_K2927006) and a spacer sequence which is specific for the desired gene to target (BBa_K3791002).


Usage, Biology and Characterization

As this part is a simplification of our final construct, usage, biology and characterization is explained there: BBa_K3791011


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]