Difference between revisions of "Part:BBa J70301:Design"
(→Design Notes) |
(→Design Notes) |
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| Line 10: | Line 10: | ||
primers: | primers: | ||
| − | caaataacggataattataaaataaaac | + | |
| − | ctatgtggttaaatatatattatcatattg | + | |
| + | GTTTCTTC GAATTC GCGGCCGC T TCTAGA G caaataacggataattataaaataaaac | ||
| + | |||
| + | GTTTCTTC CTGCAG CGGCCGC T ACTAGT A ctatgtggttaaatatatattatcatattg | ||
===Source=== | ===Source=== | ||
Revision as of 21:19, 29 March 2009
Heat shock inducible promoter for Me. florum
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Low gc content, difficult PCR primers
primers:
GTTTCTTC GAATTC GCGGCCGC T TCTAGA G caaataacggataattataaaataaaac
GTTTCTTC CTGCAG CGGCCGC T ACTAGT A ctatgtggttaaatatatattatcatattg
Source
PCR from lon protease promoter of NC_006055.