Difference between revisions of "Part:BBa K3735007"
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In order to find an appropriate expression intensity to achieve balance between metabolic burden and yields of products, NAU-CHINA designed three composite parts including three promoters with different intensity(twins). This composite part contains the promoter with middle intensity. (BBa_J23105).  
 
In order to find an appropriate expression intensity to achieve balance between metabolic burden and yields of products, NAU-CHINA designed three composite parts including three promoters with different intensity(twins). This composite part contains the promoter with middle intensity. (BBa_J23105).  
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Our model group also modeling the expression of ssuE, and found that ssuE with middle and high promoters seriously affects the respiratory metabolism of bacteria. Then we ruled out promoter of high and middle intensity, which means, low intensity promoter is suitable for gene ssuE. With promoter and RBS, the composite part can be directly imported into plasmid backbone and express ssuE gene. If you want to learn about the detailed introduction of ssuE, you can click the link (https://parts.igem.org/Part:BBa_K3735002).
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Our model group also modeling the expression of <i>ssuE</i>, and found that ssuE with middle and high promoters seriously affects the respiratory metabolism of bacteria. Then we ruled out promoter of high and middle intensity, which means, low intensity promoter is suitable for gene <i>ssuE</i>. With promoter and RBS, the composite part can be directly imported into plasmid backbone and express ssuE gene. If you want to learn about the detailed introduction of <i>ssuE</i>, you can click the link (https://parts.igem.org/Part:BBa_K3735002).
  
  

Revision as of 16:58, 18 October 2021


Coding sequence

In order to find an appropriate expression intensity to achieve balance between metabolic burden and yields of products, NAU-CHINA designed three composite parts including three promoters with different intensity(twins). This composite part contains the promoter with middle intensity. (BBa_J23105). Our model group also modeling the expression of ssuE, and found that ssuE with middle and high promoters seriously affects the respiratory metabolism of bacteria. Then we ruled out promoter of high and middle intensity, which means, low intensity promoter is suitable for gene ssuE. With promoter and RBS, the composite part can be directly imported into plasmid backbone and express ssuE gene. If you want to learn about the detailed introduction of ssuE, you can click the link (https://parts.igem.org/Part:BBa_K3735002).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 196
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]