Difference between revisions of "Part:BBa K3753011:Design"
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===References=== | ===References=== | ||
John Blazeck et al. Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters[J]. Biotechnology and Bioengineering, 2012, 109(11) : 2884-2895. | John Blazeck et al. Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters[J]. Biotechnology and Bioengineering, 2012, 109(11) : 2884-2895. | ||
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Van Slyke Ceri and Grayhack Elizabeth J.. The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region[J]. 2003, 31(15) : 4597-4607. | Van Slyke Ceri and Grayhack Elizabeth J.. The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region[J]. 2003, 31(15) : 4597-4607. | ||
Latest revision as of 16:19, 16 October 2021
UASCLB-pTEF
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 747
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 747
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 747
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 747
Illegal NgoMIV site found at 1269 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
UASCLB-pTEF is synthesized by fusing three UASCLB tandem to the tef2 promoter (BBa_K3753003). UASCLB was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The amplified product is a 240bp sequence upstream of the clb2 promoter start codon.
Source
Saccharomyces cerevisiae
References
John Blazeck et al. Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters[J]. Biotechnology and Bioengineering, 2012, 109(11) : 2884-2895.
Van Slyke Ceri and Grayhack Elizabeth J.. The essential transcription factor Reb1p interacts with the CLB2 UAS outside of the G2/M control region[J]. 2003, 31(15) : 4597-4607.