Difference between revisions of "Part:BBa K3989010"
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<partinfo>BBa_K3989010 short</partinfo> | <partinfo>BBa_K3989010 short</partinfo> | ||
| − | In this construct, outer membrane-associated protein ClyA is used to display the plant immune elicitor [[Part:BBa_K3286138|flg22]] to trigger plant immune | + | In this construct, outer membrane-associated protein ClyA is used to display the plant immune elicitor [[Part:BBa_K3286138|flg22]] to trigger plant immune responses. To make flg22 better recognized by plant immune receptor FLS2, we put long linker between ClyA and flg22 to minimize the steric hindrance. |
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| + | To detect whether ClyA can display certain plant immune elicitor to the surface of OMVs, | ||
[[File:ClyA_in_omp8_OMV.jpeg|500px]] | [[File:ClyA_in_omp8_OMV.jpeg|500px]] | ||
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[[File:omp8 OMVs on col-0.jpeg|500px]] | [[File:omp8 OMVs on col-0.jpeg|500px]] | ||
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Figure 2. | Figure 2. | ||
Revision as of 16:08, 16 October 2021
plant immune elicitor flg22 fused with ClyA for OMV display
In this construct, outer membrane-associated protein ClyA is used to display the plant immune elicitor flg22 to trigger plant immune responses. To make flg22 better recognized by plant immune receptor FLS2, we put long linker between ClyA and flg22 to minimize the steric hindrance.
To detect whether ClyA can display certain plant immune elicitor to the surface of OMVs,
Figure 1. ClyA protein with or without different PAMPs exists in outer membrane vesicles of E. coli omp8 strain.
Figure 2.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1105
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1105
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1105
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1105
Illegal NgoMIV site found at 1099
Illegal AgeI site found at 1063 - 1000COMPATIBLE WITH RFC[1000]