Difference between revisions of "Part:BBa K3989001"
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− | The three mutations are at the binding site of the protease. The catalytic efficiency of this variant and the original TEV protease is shown in Fig.1. | + | The three mutations are at the binding site of the protease. The catalytic efficiency of this variant and the original TEV protease is shown in Fig.1. More comparison between this variant and others can be found in the page of <a href="https://parts.igem.org/Part:BBa_K3989002"> BBa_K3989002</a>. |
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Revision as of 15:49, 16 October 2021
uTEV3, efficiency-enhanced TEV protease variant
uTEV3 is a mutant from the original TEV protease. Three amino acids(I138T, S153N, T180A) are mutated in this variant and the catalytic efficiency is higher than the original one and slightly lower than <a href="https://parts.igem.org/Part:BBa_K3989002"> BBa_K3989002</a>. This variant is generated by directed evolution in yeast.
Properties
The three mutations are at the binding site of the protease. The catalytic efficiency of this variant and the original TEV protease is shown in Fig.1. More comparison between this variant and others can be found in the page of BBa_K3989002.
Reference
1) Sanchez, M. I., & Ting, A. Y. (2020). Directed evolution improves the catalytic efficiency of TEV protease. Nature methods, 17(2), 167-174.