Difference between revisions of "Part:BBa K3753003"

Revision as of 15:49, 16 October 2021

pTEF2

pTEF2 is a strong constitutive promoter in Saccharomyces cerevisiae, which controls the transcription of its downstream sequence coding for translational elongation factor EF-1 α.

Characterization

Fig.1 The fluorescence intensity of GFP expressed by engineered promoters

When assembled with GFP BBa_K3112009, and CYC1 terminator BBa_K3384311 in pRS426, pTEF2 regulated the expression of GFP. Three pTEF2-based synthetic hybrid promoters were designed to enhance promoter activity. For detailed information, please visit UASCLB-pTEF BBa_K3753011, UASCIT-pTEF BBa_K3753012, UASTEF-pTEF BBa_K3753013.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 490
1000
COMPATIBLE WITH RFC[1000]

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 [[File:Gold.png|width='100%' valign='top'| |center|thumb|480*500px|''<b>Fig.1</b> The fluorescence intensity of GFP expressed by engineered promoters]]
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-When assembled with GFP BBa_K3112009, and CYC1 terminator BBa_K3384311 in pRS426, pTEF2 regulated the expression of GFP.
+When assembled with GFP <partinfo>BBa_K3112009</partinfo>, and CYC1 terminator BBa_K3384311 in pRS426, pTEF2 regulated the expression of GFP.
 Three pTEF2-based synthetic hybrid promoters were designed to enhance promoter activity. For detailed information, please visit UASCLB-pTEF <partinfo>BBa_K3753011</partinfo>, UASCIT-pTEF <partinfo>BBa_K3753012</partinfo>, UASTEF-pTEF <partinfo>BBa_K3753013</partinfo>.
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