Difference between revisions of "Part:BBa K3753013:Design"

(→‎Design Notes)
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===References===
 
===References===
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John Blazeck et al. Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters[J]. Biotechnology and Bioengineering, 2012, 109(11) : 2884-2895.

Latest revision as of 16:20, 16 October 2021


UASTEF-pTEF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 629
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 629
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 629
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 629
    Illegal NgoMIV site found at 1151
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 167
    Illegal BsaI.rc site found at 378
    Illegal BsaI.rc site found at 587


Design Notes

UASTEF-pTEF is synthesized by fusing three UASTEF tandem to the tef2 promoter (BBa_K3753003). UASTEF was obtained by PCR amplification using Saccharomyces cerevisiae BY4741 genome as template. The amplified product is a 240 bp sequence 5' of the mitotic cyclin coding gene clb2.

Source

Saccharomyces cerevisiae

References

John Blazeck et al. Controlling promoter strength and regulation in Saccharomyces cerevisiae using synthetic hybrid promoters[J]. Biotechnology and Bioengineering, 2012, 109(11) : 2884-2895.