Difference between revisions of "Part:BBa K3753012"
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<partinfo>BBa_K3753012 short</partinfo> | <partinfo>BBa_K3753012 short</partinfo> | ||
− | UAS<sub>CIT</sub>-pTEF is a synthetic hybrid promoter consisting of the upstream activation sequence(UAS), which is from the mitochondrial citrate synthase gene <em> | + | UAS<sub>CIT</sub>-pTEF is a synthetic hybrid promoter consisting of the upstream activation sequence(UAS), which is from the mitochondrial citrate synthase gene <em>cit2</em>, and the core promoter <em>tef2</em>. The tandem UAS elements of hybrid promoter could serve as synthetic transcriptional amplifiers to control expression levels. |
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Revision as of 13:35, 16 October 2021
UASCIT-pTEF
UASCIT-pTEF is a synthetic hybrid promoter consisting of the upstream activation sequence(UAS), which is from the mitochondrial citrate synthase gene cit2, and the core promoter tef2. The tandem UAS elements of hybrid promoter could serve as synthetic transcriptional amplifiers to control expression levels.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 845
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 845
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 845
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 845
Illegal NgoMIV site found at 1367 - 1000COMPATIBLE WITH RFC[1000]