Difference between revisions of "Part:BBa K3753011"
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− | + | UAS<sub>CLB-pTEF is a synthetic hybrid promoter consisting of the upstream activation sequence(UAS), which is from the mitotic cyclin coding gene clb2, and the core promoter TEF2. The tandem UAS elements of hybrid promoter could serve as synthetic transcriptional amplifiers to control expression levels. | |
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Revision as of 13:17, 16 October 2021
UASCLB-pTEF
UASCLB-pTEF is a synthetic hybrid promoter consisting of the upstream activation sequence(UAS), which is from the mitotic cyclin coding gene clb2, and the core promoter TEF2. The tandem UAS elements of hybrid promoter could serve as synthetic transcriptional amplifiers to control expression levels.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 747
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 747
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 747
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 747
Illegal NgoMIV site found at 1269 - 1000COMPATIBLE WITH RFC[1000]