Difference between revisions of "Part:BBa K3846135"
 
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<partinfo>BBa_K3846135 short</partinfo>
 
<partinfo>BBa_K3846135 short</partinfo>
  
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Parthenolide synthase (CYP71CA1) - phytobrick
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This part contains the codon-optimised coding sequence of Costunolide synthase (CYP71BL2) from <i> Tanacetum parthenium</i>. The enzyme is involved in the biosynthesis of germacrene-derived sesquiterpene lactones and component of the parthenolide biosynthetic pathway. It catalyzes the conversion of costunolide to parthenolide.
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The part was modified by removing an N-terminal (30 aa) and an internal (301-321 aa) transmembrane domain.
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<b>5' fusion site:</b> ATCG
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<b>3' fusion site:</b> GCTT
  
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without transmembran domain (aa 1-30, 301-321)
 
  
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ATCG-GCTT
 
  
 
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<!-- Add more about the biology of this part here
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<partinfo>BBa_K3846135 parameters</partinfo>
 
<partinfo>BBa_K3846135 parameters</partinfo>
 
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===iGEM Hamburg 2021 part collection===
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Terpenoids are an important group of natural products used as biofuels, drugs or fragrances.  Naturally occuring in plants it has been shown that microbial terpene production in microorganisms like yeast, E. coli or  cyanobacteria is possible.
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Nevertheless iGEM projects seem to rarely focus on this interesting class of natural products which is correlated with a lack of useful parts inside the iGEM registry.
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Fortunately we were able to change that and designed a novel golden gate based toolbox which allows.
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<ol style="list-style-type:lower-alpha">
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  <li>production of terpenoid precursors and simple terpenoids</li>
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  <li>creation of CYP P450-reductase fusion enzymes to optimise processing of terpenoid precursors and production of bioactive target products</li>
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  <li>modularity of the system to enable exchange of linker sequences/promoters/etc. (MoClo-compatible toolbox)</li>
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</ol>
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===MoClo-based Part Design 2.0===
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<p>To improve the usefulness of our parts, we then aimed to make our parts compatible with the MoClo standard of goten gate based IIS restriction enzyme assembly. Thereby we expanded the Common Genetic Syntax for fusion sites to allow the creation of a) fusion proteins connected by linker sequences and b) multiple CDS expressed in an operon.
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More useful information and an overview of all our parts can be found on our [https://2021.igem.org/Team:Hamburg/Part_Collection wiki].
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[[File:T--Hamburg--parts overview MolClo.png|600px|thumb|left|'''Figure 1''': <b> MoClo syntax of the part collection. </b> <br>]]

Latest revision as of 21:18, 19 October 2021


Parthenolide synthase (CYP71CA1) - phytobrick

This part contains the codon-optimised coding sequence of Costunolide synthase (CYP71BL2) from Tanacetum parthenium. The enzyme is involved in the biosynthesis of germacrene-derived sesquiterpene lactones and component of the parthenolide biosynthetic pathway. It catalyzes the conversion of costunolide to parthenolide.

The part was modified by removing an N-terminal (30 aa) and an internal (301-321 aa) transmembrane domain.

5' fusion site: ATCG

3' fusion site: GCTT


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



iGEM Hamburg 2021 part collection

Terpenoids are an important group of natural products used as biofuels, drugs or fragrances. Naturally occuring in plants it has been shown that microbial terpene production in microorganisms like yeast, E. coli or cyanobacteria is possible. Nevertheless iGEM projects seem to rarely focus on this interesting class of natural products which is correlated with a lack of useful parts inside the iGEM registry.

Fortunately we were able to change that and designed a novel golden gate based toolbox which allows.

  1. production of terpenoid precursors and simple terpenoids
  2. creation of CYP P450-reductase fusion enzymes to optimise processing of terpenoid precursors and production of bioactive target products
  3. modularity of the system to enable exchange of linker sequences/promoters/etc. (MoClo-compatible toolbox)

MoClo-based Part Design 2.0

To improve the usefulness of our parts, we then aimed to make our parts compatible with the MoClo standard of goten gate based IIS restriction enzyme assembly. Thereby we expanded the Common Genetic Syntax for fusion sites to allow the creation of a) fusion proteins connected by linker sequences and b) multiple CDS expressed in an operon. More useful information and an overview of all our parts can be found on our wiki.

Figure 1: MoClo syntax of the part collection.