Difference between revisions of "Part:BBa K3776001"

Revision as of 10:35, 16 October 2021

DavB

DavB, L-Lysine monooxygenase, catalyzes the reaction of L-lysine to 5-aminovaleramide in the natural 5-aminovalerate pathway in Pseudomonas putida KT2440. This DavB sequence has been codon optimized for expression in Synechococcus elongatus UTEX 2973.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NotI site found at 239
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1328
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1488
Illegal AgeI site found at 895
Illegal AgeI site found at 1144
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K3776001 short</partinfo>
-Designed by Vo et al. (2020), this protein complex includes transposases TnsA, TnsB, TnsC and Cas enzymes Cas 8, Cas7, Cas6. These proteins are guided by a crRNA prefix flanked by two repeats. Users should clone their crRNA between these two repeats. This RNA guided protein complex inserts a donor DNA located between two flanks behind the into the genome of the host organism. Users should clone their genes of interest between these two flanks.
+DavB, L-Lysine monooxygenase, catalyzes the reaction of L-lysine to 5-aminovaleramide in the natural 5-aminovalerate pathway in Pseudomonas putida KT2440. This DavB sequence has been codon optimized for expression in Synechococcus elongatus UTEX 2973.
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