Difference between revisions of "Part:BBa K3989002"
(→Structure and binding site) |
(→Structure and binding site) |
||
Line 11: | Line 11: | ||
<img src="https://static.igem.org/mediawiki/parts/2/2b/21_UZurich_eTEV_binding_site.jpeg"> | <img src="https://static.igem.org/mediawiki/parts/2/2b/21_UZurich_eTEV_binding_site.jpeg"> | ||
<figcaption><b>Fig. 1 </b>. - Sub-structure at the binding site of eTEV. The blue amino acids are mutated and the purple one represents the cut site amino acid residue</figcaption> | <figcaption><b>Fig. 1 </b>. - Sub-structure at the binding site of eTEV. The blue amino acids are mutated and the purple one represents the cut site amino acid residue</figcaption> | ||
+ | <figure> | ||
<!-- --> | <!-- --> |
Revision as of 09:37, 16 October 2021
eTEV, efficiency-enhanced TEV protease variant
eTEV protease is mutant from the original TEV protease. Seven amino acids(S3I, P8Q, S31T, E79G, T173A, V219R, A231V) are mutated in this variant and the catalytic efficiency is 2-fold higher than the original one.
Structure and binding site
<figure>
<img src=""> <figcaption>Fig. 1 . - Sub-structure at the binding site of eTEV. The blue amino acids are mutated and the purple one represents the cut site amino acid residue</figcaption>
<figure>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
1) Denard, C. A., Paresi, C., Yaghi, R., McGinnis, N., Bennett, Z., Yi, L., ... & Iverson, B. L. (2021). YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants. ACS Synthetic Biology, 10(1), 63-71.