Difference between revisions of "Part:BBa K3866038"

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===Verification of Cloning===
 
===Verification of Cloning===
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[[File:T--Thessaly--gfpgel.png|800px|thumb|none|<i><b>Figure 2.</b>: (C=Cut, U=Uncut) Restriction digestion of α1-sbm with: EcoRI, Expected bands: 6345bp, 2947bp, 597bp, Positive result: C2</i>]]
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[[File:T--Thessaly--gfpgel.png|800px|thumb|none|<i><b>Figure 2.</b>: (C=Cut, U=Uncut) Restriction digestion of α-sfGFP TU with: EcoRI, Expected bands: 2847bp, 801bp, 146bp, Positive result: C3, C4, C5</i>]]
  
 
===Experimental Use and Experience===
 
===Experimental Use and Experience===

Revision as of 09:29, 16 October 2021


J23115:sfGFP:Terminator

Usage and Biology

This TU includes the sfGFP gene placed under the control of the constitutive Anderson promoter J23115 BBa_J23115.

Design Notes

The coding sequence was domesticated. We removed BsmBI and BsaI sites in order to be compatible with GoldenBraid and MoClo.

Figure 1. The level α module of the sfGFP Translational Unit: α1: J23115:RBS-sfGFP-Double terminator

Verification of Cloning

Figure 2.: (C=Cut, U=Uncut) Restriction digestion of α-sfGFP TU with: EcoRI, Expected bands: 2847bp, 801bp, 146bp, Positive result: C3, C4, C5

Experimental Use and Experience

This part showed functionality at the following parts: BBa_K3866029, BBa_K3866031

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 192
  • 1000
    COMPATIBLE WITH RFC[1000]