Difference between revisions of "Part:BBa K3815008"
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<partinfo>BBa_K3815037 short</partinfo> | <partinfo>BBa_K3815037 short</partinfo> | ||
| − | + | This part is made of Hist tag for the peptide production. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the purpose protein is LL37 derived from human. This is antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water. | |
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Revision as of 09:35, 17 October 2021
No part name specified with partinfo tag.
This part is made of Hist tag for the peptide production. After producing peptide with His tag, it is recovered by Ni chromatography. Finally, adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the purpose protein is LL37 derived from human. This is antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.
Sequence and Features No part name specified with partinfo tag.