Difference between revisions of "Part:BBa K3989002:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | Single mutation from the original TEV protease. | |
− | + | ||
− | + | ||
===Source=== | ===Source=== | ||
− | + | Original TEV protease sequence and structure. PDB: 1LVM | |
===References=== | ===References=== |
Revision as of 09:14, 16 October 2021
eTEV, efficiency-enhanced TEV protease variant
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Single mutation from the original TEV protease.
Source
Original TEV protease sequence and structure. PDB: 1LVM
References
1) Denard, C. A., Paresi, C., Yaghi, R., McGinnis, N., Bennett, Z., Yi, L., ... & Iverson, B. L. (2021). YESS 2.0, a Tunable Platform for Enzyme Evolution, Yields Highly Active TEV Protease Variants. ACS Synthetic Biology, 10(1), 63-71.