Difference between revisions of "Part:BBa K3788002:Experience"
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https://2021.igem.org/wiki/images/a/a3/T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png | https://2021.igem.org/wiki/images/a/a3/T--Aix-Marseille--DNA-SLIC-Amplification-xtoxtox.png | ||
<p><u>Figure</u>. <b>Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning.</b> <i> | <p><u>Figure</u>. <b>Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning.</b> <i> | ||
| − | a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 | + | a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 bp), flagcry11Aa (expected 1847 bp), strepcyt1Aa (expected 775 bp) and 6hisp20 (expected 568 bp); L (SMART Ladder, its size is shown on the right side). </i></p> |
Revision as of 16:57, 15 October 2021
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Applications of BBa_K3788002
PCR amplification of Ghisp20 gene :
The DNA sequence of Ghisp20 was amplify by PCR Q5 to generate fragment allowing a SLIC cloning. The expected size is 570bp and a +/- 600bp band is observed. Amplification of DNAs sequences with primers were validated.
Figure. Verification PCR amplification of DNAs sequence and these extensions for SLIC cloning. a. Control – (Absence of DNAs, no band expected); b, c, d, e. Amplification of 6hisp20_flagcry11Aa (expected 2500 bp), flagcry11Aa (expected 1847 bp), strepcyt1Aa (expected 775 bp) and 6hisp20 (expected 568 bp); L (SMART Ladder, its size is shown on the right side).
User Reviews
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