Difference between revisions of "Part:BBa K3930023"
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<h3>Production of &alpha;-ionone (BBa_K3930003)</h3>
 
<h3>Production of &alpha;-ionone (BBa_K3930003)</h3>
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<p> The part (BBa_K3930003) was linearized and transformed into the <i>Sacharomyces.cerevisiae</i> LycoYeast strain. The production of &alpha;-ionone was induced by plating the transformants onto YPD media containing 20mg.ml-1 of galactose. Figure 1 shows the mass spectra between an induced and a non-induced strain lysate.</p>
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<p> The part (BBa_K3930003) was linearized and transformed into the <i>Sacharomyces.cerevisiae</i> LycoYeast strain. The production of &alpha;-ionone was induced by plating the transformants onto YPD media containing 20mg.ml-1 of galactose. Figure 1 shows the GC-MS mass spectra between lysates from induced and non-induced strain lysate.</p>
 
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                     <a href="https://2021.igem.org/wiki/images/c/c7/T--Toulouse_INSA-UPS--2021_fig37_results_LycoYeast-FRAMBOISE-notfused.png" class="internal" title="Enlarge"></a>
 
                     <a href="https://2021.igem.org/wiki/images/c/c7/T--Toulouse_INSA-UPS--2021_fig37_results_LycoYeast-FRAMBOISE-notfused.png" class="internal" title="Enlarge"></a>
 
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                 <b>Figure 1: </b> <b> Production of &alpha;-ionone upon galactose activation</b>
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                 <b>Figure 1: </b> <b> Production of &alpha;-ionone upon galactose induction</b>
 
                 <p>α-ionone is produced in vivo by our strain when it is induced by galactose. On the right are presented the mass spectra that correspond between the standard and the observed peak</p>
 
                 <p>α-ionone is produced in vivo by our strain when it is induced by galactose. On the right are presented the mass spectra that correspond between the standard and the observed peak</p>
 
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<p><b>This promoter gal without the kozak sequence work under those lab conditions.</b><p>
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<p><b>This pGAL1 promoter without Kozak sequence is functional under these lab conditions.</b><p>
 
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Revision as of 13:48, 15 October 2021


Galactose inducible promoter Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 70
  • 1000
    COMPATIBLE WITH RFC[1000]


Introduction

The promoter Gal1 is a galactose inducible promoter. This sequence comes from Sacharomyces cerevisiae genome. This part is related to the extensively used and characterized part BBa_J63006. Nonetheless, our promoter lack the kozak sequence.

Results

Production of α-ionone (BBa_K3930003)

The part (BBa_K3930003) was linearized and transformed into the Sacharomyces.cerevisiae LycoYeast strain. The production of α-ionone was induced by plating the transformants onto YPD media containing 20mg.ml-1 of galactose. Figure 1 shows the GC-MS mass spectra between lysates from induced and non-induced strain lysate.



Figure 1: Production of α-ionone upon galactose induction

α-ionone is produced in vivo by our strain when it is induced by galactose. On the right are presented the mass spectra that correspond between the standard and the observed peak


This pGAL1 promoter without Kozak sequence is functional under these lab conditions.