Difference between revisions of "Part:BBa K3930032"
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<h2>Introduction</h2>
 
<h2>Introduction</h2>
 
<p>The SpecR cassette is composed of the promoter PaadA and the resistance gene <i>specR (aadA)</i>. The resistance gene is based on part (BBa_K1323012), but it was codon optimized for expression into <i>S.elongatus</i>.</p>
 
<p>The SpecR cassette is composed of the promoter PaadA and the resistance gene <i>specR (aadA)</i>. The resistance gene is based on part (BBa_K1323012), but it was codon optimized for expression into <i>S.elongatus</i>.</p>
−
<p>This gene codes for an aAminoglycoside adenylyltransferase , which inactivates spectinomycin by adding a adenyl group. Therefore, this cassette allows the selection of yeast transformants upon addition of spectinomycin into the media. </p>  
+
<p>This gene codes for an aminoglycoside adenylyltransferase , which inactivates spectinomycin by adding a adenyl group. Therefore, this cassette allows the selection of yeast transformants upon addition of spectinomycin into the media. </p>  
  
 
<h3>Part characterization</h3>
 
<h3>Part characterization</h3>

Revision as of 12:49, 15 October 2021


Spectinomycine selective cassette Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1032
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 7

Introduction

The SpecR cassette is composed of the promoter PaadA and the resistance gene specR (aadA). The resistance gene is based on part (BBa_K1323012), but it was codon optimized for expression into S.elongatus.

This gene codes for an aminoglycoside adenylyltransferase , which inactivates spectinomycin by adding a adenyl group. Therefore, this cassette allows the selection of yeast transformants upon addition of spectinomycin into the media.

Part characterization

The part (BBa_K3930032) was used to select for genomic integration of part (BBa_K3930026). Transformants were plated onto BG11 Petri plates containing 10 μg.ml-1 spectinomycin. The Toulouse INSA UPS team managed to select transformants with this selection marker (for more details, check the (BBa_K3930026) part page)