Difference between revisions of "Part:BBa K3733005"
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LThe strength of this promoter is much weaker than J23106. </p> | LThe strength of this promoter is much weaker than J23106. </p> | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
<p>J23106 is a medium-strength constitutive promoter of the Anderson family. By editing J23106's -35 region by replacing nucleotides 3-5 from TAC to ATA, J23106a was successfully constructed (<b>Figure 1</b>),which is a very weak promoter. </p> | <p>J23106 is a medium-strength constitutive promoter of the Anderson family. By editing J23106's -35 region by replacing nucleotides 3-5 from TAC to ATA, J23106a was successfully constructed (<b>Figure 1</b>),which is a very weak promoter. </p> | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3733005 parameters</partinfo> | <partinfo>BBa_K3733005 parameters</partinfo> | ||
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Revision as of 14:28, 15 October 2021
J23106a:Improved promoter of J23106
J23106a is a constitutive promoter obtained by mutating J23106.(https://parts.igem.org/Part:BBa_J23106) LThe strength of this promoter is much weaker than J23106.
Usage and Biology
J23106 is a medium-strength constitutive promoter of the Anderson family. By editing J23106's -35 region by replacing nucleotides 3-5 from TAC to ATA, J23106a was successfully constructed (Figure 1),which is a very weak promoter.
Functional Parameters
To characterize this part, J23106a and J23106 were cloned into pSC101 vector separately. We chose neGFP(https://parts.igem.org/Part:BBa_K3733012) as the reporter. Plasmids were transferred into E.coli DH5α. The E. coli strain was cultured overnight, and the relative intensity of J23106a was evaluated by measuring RFU/OD600 under the control of two different promoters. The relative fluorescence unit (RFU) was determined by Synergy H1 microplate reader.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
biology | Escherichia coli |