Difference between revisions of "Part:BBa K3982038:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | Parts from [https://parts.igem.org/Part:BBa_K3982030 | + | Parts from [https://parts.igem.org/Part:BBa_K3982030 CODE M Construct C2] assembled with T7 promoter, RBS and T7TE terminator. |
===Source=== | ===Source=== | ||
| − | [https://www.addgene.org/15030 | + | [https://www.addgene.org/15030/ Addgene #15030] |
===References=== | ===References=== | ||
Latest revision as of 15:37, 21 October 2021
CODE M Construct S2
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 406
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Parts from CODE M Construct C2 assembled with T7 promoter, RBS and T7TE terminator.
Source
References
1) Kim, D.Y., Lee, J.M., Moon, S.B. et al. Efficient CRISPR editing with a hypercompact Cas12f1 and engineered guide RNAs delivered by adeno-associated virus. Nat Biotechnol (2021). https://doi.org/10.1038/s41587-021-01009-z
2) Harrington, L. B., Burstein, D., Chen, J. S., Paez-Espino, D., Ma, E., Witte, I. P., Cofsky, J. C., Kyrpides, N. C., Banfield, J. F., & Doudna, J. A. (2018). Programmed DNA destruction by miniature CRISPR-Cas14 enzymes. Science (New York, N.Y.), 362(6416), 839–842. https://doi.org/10.1126/science.aav4294