Difference between revisions of "Part:BBa K3930008"
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<h2>Introduction</h2> | <h2>Introduction</h2> | ||
<p>This sequence codes for the Linalool synthase, an enzyme that transform GPP into linalool, a molecule which smell like flower. The sequence derived from the <i>Lavandula angustifolia</i> genome. We take advantage of the publication of (Amiri et al. 2016). | <p>This sequence codes for the Linalool synthase, an enzyme that transform GPP into linalool, a molecule which smell like flower. The sequence derived from the <i>Lavandula angustifolia</i> genome. We take advantage of the publication of (Amiri et al. 2016). | ||
− | <p>There is already BBa_K3052002 on the registry for linalool synthase. This part is codon optimized for translation in E. coli, but ours is codon optimized for translation in S. cerevisiae.</p> | + | <p>There is already BBa_K3052002 on the registry for linalool synthase. This part is codon optimized for translation in <i>E. coli</i>, but ours is codon optimized for translation in <i>S. cerevisiae</i>.</p> |
Revision as of 21:09, 14 October 2021
Gene coding for the Linalool synthase
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1135
Illegal PstI site found at 23 - 1000COMPATIBLE WITH RFC[1000]
Introduction
This sequence codes for the Linalool synthase, an enzyme that transform GPP into linalool, a molecule which smell like flower. The sequence derived from the Lavandula angustifolia genome. We take advantage of the publication of (Amiri et al. 2016).
There is already BBa_K3052002 on the registry for linalool synthase. This part is codon optimized for translation in E. coli, but ours is codon optimized for translation in S. cerevisiae.
References
- Amiri P, Shahpiri A, Asadollahi MA, Momenbeik F, Partow S. 2016. Metabolic engineering of Saccharomyces cerevisiae for linalool production. Biotechnol Lett. 38(3):503–508. doi:10.1007/s10529-015-2000-4.