Difference between revisions of "Part:BBa K3962339"
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J23100 constitutive promoter is ligated to mCherry for the constant expression of fluorescent proteins. In our project, we used this construct as reference to calibrate the inducibility of L-arabinose on pBAD promoter. | J23100 constitutive promoter is ligated to mCherry for the constant expression of fluorescent proteins. In our project, we used this construct as reference to calibrate the inducibility of L-arabinose on pBAD promoter. | ||
| − | [[Image:T-Leiden-parts-Figuer1.png]] | + | [[Image:T-Leiden-parts-Figuer1.png]|50px] |
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Revision as of 14:29, 13 October 2021
J23100 promoter with mCherry for constant expression
J23100 constitutive promoter is ligated to mCherry for the constant expression of fluorescent proteins. In our project, we used this construct as reference to calibrate the inducibility of L-arabinose on pBAD promoter.
[[Image:T-Leiden-parts-Figuer1.png]|50px]
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]